Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
Frizzled 8 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Frizzled 8 antibody was raised against an 11 amino acid synthetic peptide near the C-Terminus of Frizzled 8.
FZD8
Reactivity: Human, Mouse, Rat, Dog
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 75 kDa band observed in human heart and human pancreas lysates (calculated MW of 73.3 kDa according to NP_114072.1). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human pancreas, shows granular cytoplasmic staining of acinar cells. Cells of the Islets of Langerhans are weakly stained, if at all. Recommended concentration, 3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.