This antibody is expected to recognize both reported isoforms (NP_057412.1 and NP_005676.2).
Purification
GTF2IRD1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
GTF2IRD1 antibody was raised against a 14 amino acid synthetic peptide near the internal region of GTF2IRD1.
GTF2IRD1
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 30 kDa and 40 kDa in human heart lysate after 0.3 μg/mL antibody staining. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
GTF2IRD1
(General Transcription Factor II I Repeat Domain-Containing 1 (GTF2IRD1))