IGFBP6 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
IGFBP6 antibody was raised against a 14 amino acid synthetic peptide near the internal region of IGFBP6.
IGFBP6
Reactivity: Human
WB, IHC, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 25 kDa band observed in human adrenal gland lysates (calculated MW of 25.3 kDa according to NP_002169.1). In transfected HEK293 transiently expressing IGFBP6 a band of approximately 29 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
IGFBP6
(Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))