MAD4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
MAD4 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of MAD4.
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 22 kDa band observed in human kidney lysates (predicted MW of 24 kDa according to NP_006445). Recommended for use at 0.5-2 μg/mL. Please note the appearance of this band was blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.