Midline 2 antibody (C-Term)

Catalog No. ABIN2465946

Product Details anti-Midline 2 Antibody

Target: Midline 2 (MID2)

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Midline 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: This antibody is expected to recognize both human isoforms of this protein.

Purification: MID2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: MID2 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of MID2.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 80 kDa band observed in mouse heart lysates (calculated MW of 81.3 kDa according to NP_036348.1 and 77.9 kDa according to NP_438112.1). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules. Recommended concentration, 5-10 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for Midline 2

Target: Midline 2 (MID2)

Alternative Name: MID2 (MID2 Products)

Synonyms: MID2 antibody, fxy2 antibody, rnf60 antibody, trim1 antibody, FXY2 antibody, RNF60 antibody, TRIM1 antibody, Trim1 antibody, RGD1565674 antibody, midline 2 antibody, midline 2 L homeolog antibody, MID2 antibody, mid2 antibody, mid2.L antibody, Mid2 antibody

Gene ID: 11043

NCBI Accession: NP_036348, NP_438112

UniProt: Q9UJV3