MURF1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
MURF1 antibody was raised against a 13 amino acid synthetic peptide near the N-Terminus of MURF1.
TRIM63
Reactivity: Human
WB
Host: Mouse
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 60 kDa band in human skeletal muscle lysates after 0.1 μg/mL antibody staining.2 kDa according to NP_115977.2. The 60 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human skeletal muscle shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.