Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
PEBP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PEBP1 antibody was raised against a 14 amino acid synthetic peptide near the internal region of PEBP1.
PEBP1
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
1D3-1B6
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 21 kDa band observed in human prostate lysates (calculated MW of 21.1 kDa according to NP_002558.1). Recommended concentration: 0.01-0.03 μg/mL. Immunohistochemistry: In paraffin embedded adrenal gland shows strong stinaing of secretory cells. Recommended concentration, 2-5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
PEBP1
(Phosphatidylethanolamine Binding Protein 1 (PEBP1))