Prefoldin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Prefoldin antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of Prefoldin.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments (at 0.2 μg/mL) gave a strong band at approximately 20-25 kDa band in human muscle lysates. Approximately 14 kDa band observed in K562 lysate (calculated MW of 14.2 kDa according to NP_002613.2). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 37 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.