PRODH antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PRODH antibody was raised against a 15 amino acid synthetic peptide near the internal region of PRODH.
PRODH
Reactivity: Human, Mouse, Rat
IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 85 kDa and 25 kDa in human brain lysates after 1 μg/mL antibody staining.0 kDa according to NP_057419.2. Both detected bands were successfully blocked by incubation with the immunizing peptide. Immunohistochemistry In paraffin embedded cerebellum shows mitochondrial staining in smaller neurons. Recommended concentration, 3-6 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.