Retinoic Acid Receptor alpha antibody (C-Term)

Catalog No. ABIN2466181

Product Details anti-Retinoic Acid Receptor alpha Antibody

Target: Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Retinoic Acid Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity: NP_000955.1 (isfa) and NP_001028775.1(alpha) represent identical proteins. This antibody is expected to recognize all three reported isoforms (NP_000955.1, NP_001019980.1, NP_001028775.1). Please note the epitope is from the mouse/rat sequence. Human sequence is SPSLSPSSNRSSPATHSP and thus highly similar.

Purification: RARA antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: RARA antibody was raised against an 18 amino acid synthetic peptide near the C-Terminus of RARA.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 50+48 kDa bands observed in human brain lysates (calculated MW of 50.8 kDa according to NP_000955.1, NP_001019980.1 and NP_001028775.1). Recommended concentration: 0.03-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human cerebellum shows staining of Purkinje cytoplasm. Recommended concentration, 4-6 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for Retinoic Acid Receptor alpha

Target: Retinoic Acid Receptor alpha (RARA) (Retinoic Acid Receptor, alpha (RARA))

Alternative Name: RARA (RARA Products)

Synonyms: NR1B1 antibody, RAR antibody, Nr1b1 antibody, RARalpha1 antibody, rar-alpha antibody, etID309833.12 antibody, rara antibody, rara2a antibody, zRAR antibody, zRAR-alpha antibody, zgc:109797 antibody, nr1b1 antibody, RAR-ALPHA1 antibody, HS-RARa antibody, fj66e06 antibody, rara2b antibody, wu:fj66e06 antibody, retinoic acid receptor alpha antibody, retinoic acid receptor, alpha antibody, retinoic acid receptor, alpha a antibody, retinoic acid receptor alpha L homeolog antibody, retinoic acid receptor, alpha b antibody, RARA antibody, Rara antibody, LOC100136372 antibody, raraa antibody, rara.L antibody, rara antibody, rarab antibody

Gene ID: 5914

NCBI Accession: NP_000955, NP_001019980, NP_001028775

UniProt: P10276

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, S100 Proteins