This Retinoid X Receptor gamma antibody is un-conjugated
Application
Western Blotting (WB), ELISA
Specificity
This antibody is expected to recognize an epitope corresponding to aa 213-226 of human RXR gamma protein (hinge region) and does not cross-react with either RXR alpha or beta.
Purification
RXR gamma antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
RXR gamma antibody was raised against a 14 amino acid synthetic peptide near the internal region of RXR gamma.
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 48 kDa band observed in human brain lysates (calculated MW of 50.9 kDa according to NP_008848). Recommended concentration: 0.3-2 μg/mL. Some bands of unknown identity were also consistently observed around 37 kDa . These bands were successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
Retinoid X Receptor gamma (RXRG)
(Retinoid X Receptor, gamma (RXRG))