Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Specificity
Both variants (NP_002952.1, NP_062427.1) represent identical protein.
Purification
S100A4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
S100A4 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of S100A4.
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 13 kDa band observed in human placenta lysates (calculated MW of 11.7 kDa according to NP_002952.1and NP_062427.1). Recommended concentration: 0.03-0.1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
s100a4 (S100A4)
(S100 Calcium Binding Protein A4 (S100A4))