The peptide sequence used (from AAC50460.1) differs by one residue from the RefSeq entry (NP_003002.1) and the three other Genbank protein sequences referenced in the Locuslink entry. It is not known whether this difference is due to a sequencing error or polymophism but the antibody is expected to recognize both sequences.
Purification
SET antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SET antibody was raised against a 14 amino acid synthetic peptide near the N-Terminus of SET.
SET
Reactivity: Human, Mouse, Rat
WB, FACS, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:1000. Western Blot: Approximately 20-25 kDa and 40 kDa bands seen in human kidney extracts. Recommended for use at 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.