SH2D1A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SH2D1A antibody was raised against a 10 amino acid synthetic peptide near the internal region of SH2D1A.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 16 kDa band observed in human thymus lysates (calculated MW of 14.2x kDa according to NP_002342.1). In transfected HEK293 transiently expressing SH2D1A a band of approximately 16 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.