Both variants represent identical protein (NP_694985.28 and NP_006738.2).
Purification
SIPA1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SIPA1 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of SIPA1.
SIPA1
Reactivity: Human
WB, IF, EIA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Approximately 150 kDa band observed in human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112 kDa according to NP_694985.28 and NP_006738.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31,272 (44) :28081-8, PMID: 9346962). Recommended concentration: 1-3 μg/mL. an additional band of 55-60 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this antibody is used for other assays than Western Blot.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.