This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
Purification
GAD67 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
GAD67 antibody was raised against a 12 amino acid synthetic peptide near the internal region of GAD67.
GAD1
Reactivity: Human, Mouse, Rat
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 70 kDa band observed in human cerebellum and in mouse and rat brain lysates (calculated MW of 66.9 kDa according to human NP_000808.2 and 66.6 kDa according to mouse NP_032103.2). Recommended concentration: 0.3-1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.