Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
GABRA4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
GABRA4 antibody was raised against a 13 amino acid synthetic peptide near the internal region of GABRA4.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 70 kDa band observed in human brain (cerebellum and frontal cortex) lysates (calculated MW of 61.6 kDa according to NP_000800.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human cortex shows patchy staining near or at the neuronal cell membrane. Recommended concentration, 2-4 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
GABRA4
(gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4))