Calcineurin A antibody (AA 264-283) (FITC)
-
- Target See all Calcineurin A (CAN) Antibodies
- Calcineurin A (CAN)
-
Binding Specificity
- AA 264-283
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Calcineurin A antibody is conjugated to FITC
-
Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- Detects ~61 kDa.
- Cross-Reactivity
- Dog, Human, Mouse, Rabbit, Rat
- Purification
- Peptide Affinity Purified
- Immunogen
- Human Calcineurin A peptide (AA 264-283)
- Top Product
- Discover our top product CAN Primary Antibody
-
-
- Application Notes
-
- WB (1:1000)
- ICC/IF (1:120)
- IHC (1:100)
- optimal dilutions for assays should be determined by the user.
- Comment
-
1 μg/ml of ABIN2481985 was sufficient for detection of Calcineurin A in 20 μg of Heat shock Hela lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
-
- Target
- Calcineurin A (CAN)
- Alternative Name
- Calcineurin A (CAN Products)
- Synonyms
- Cna antibody, DDBDRAFT_0185021 antibody, DDBDRAFT_0217855 antibody, DDB_0185021 antibody, DDB_0217855 antibody, PPP3CC antibody, CAN A1/PP2B antibody, CG1455 antibody, CNA1 antibody, Can antibody, Cana1 antibody, CnA antibody, CnnA21EF antibody, D14 antibody, DmCanA1-100B antibody, Dmel\\CG1455 antibody, PP2B antibody, PP2B 21EF antibody, PpD14 antibody, canA antibody, pMY4 antibody, calcineurin A antibody, Calcineurin A antibody, CaM-dependent protein phosphatase, catalytic subunit antibody, protein phosphatase 3 catalytic subunit alpha antibody, Calcineurin A1 antibody, protein phosphatase 3 catalytic subunit beta antibody, Cna antibody, NFIA_077970 antibody, ACLA_011200 antibody, PAS_chr3_0414 antibody, canA antibody, PPP3CA antibody, CanA1 antibody, PPP3CB antibody
- Background
- Calcineurin is a heterodimeric phosphatase protein, also known as calcium-dependent serine-threonine phosphatase. The structure consists of a catalytic subunit alpha, Calcineurin A (57-59KDa) the active site and a Ca2+ binding unit, Calcineurin B (19-20KDa) the regulatory subunit(1). Calcineurin plays a key role in the T-cell response growth and differentiation mechanism, regulating the activation of the Nuclear factor of activated T-cells (NFATc) which are important in the expression of IL-2 genes. Calcineurin has been the target of inhibitors, the novel and structural immune-suppressants antifungal drugs(2). Genetic studies in yeast and fungi established the molecular basis of the inhibition mechanism by cyclosporine A and FK506 (3).
- Gene ID
- 5530
- UniProt
- Q08209
-