Amyloid antibody (Biotin)
-
- Target
- Amyloid
- Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- Biotin
-
Application
- ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- Recognizes all types of amyloid oligomers. Appears to recog a peptide backbone epitope common to amyloid oligomers, but not found in native proteins, amyloidogenic monomer or mature amyloid fibrils.
- Cross-Reactivity
- Eukaryotes, Human, Mouse, Rat
- Purification
- Protein A Purified
- Immunogen
- Synthetic molecular mimic of soluble oligomers
-
-
- Application Notes
-
- WB (1:200)
- IP (1:200)
- optimal dilutions for assays should be determined by the user.
- Comment
-
A 1:200 dilution of ABIN2486478 was sufficient for detection of amyloid oligomers in 10 μg of mouse brain lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
-
- Target
- Amyloid
- Synonyms
- amyloid beta precursor protein antibody, App antibody
- Background
- Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres (1, 2). These include the amyloid-β peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer's symptomology, the deposition of α-synuclein in the Lewy bodies of Parkinson's disease, and accumulation of polyglutamine-containing aggregates in Huntington's disease (2, 3).
- Gene ID
- 8666
- NCBI Accession
- NM_000484
- UniProt
- P05067
-