The Antibody detects endogenous levels of ATF-4 only when phosphorylated at Serine 219.
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Immunogen
ATF-4 (phospho Ser219) antibody was raised against a peptide sequence around phosphorylation site of Serine 219 (N-D-S (p)-G-T) derived from Human ATF-4.
ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.