BTK antibody (pTyr223)

Catalog No. ABIN2567065

Product Details anti-BTK Antibody

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Binding Specificity: pTyr223

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BTK antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody detects endogenous levels of BTK only when phosphorylated at tyrosine 223.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: BTK (phospho Tyr223) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 223 (A-L-Y (p)-D-Y) derived from Human BTK.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for BTK

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Alternative Name: BTK (BTK Products)

Synonyms: BTK antibody, atk antibody, bpk antibody, xla antibody, imd1 antibody, agmx1 antibody, psctk1 antibody, AGMX1 antibody, AT antibody, ATK antibody, BPK antibody, IMD1 antibody, PSCTK1 antibody, XLA antibody, AI528679 antibody, xid antibody, Bruton tyrosine kinase antibody, Bruton agammaglobulinemia tyrosine kinase antibody, BTK antibody, btk antibody, Btk antibody

Background: Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK.

Molecular Weight: 80 kDa

Gene ID: 695

NCBI Accession: NP_000052

UniProt: Q06187

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling