The antibody detects endogenous levels of Claudin 3 only when phosphorylated at tyrosine 219.
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Immunogen
Claudin 3 (phospho Tyr219) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 219 (R-K-D-Y (p)-V) derived from Human Claudin 3.
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands.