TNF alpha antibody (AA 77-233)
Quick Overview for TNF alpha antibody (AA 77-233) (ABIN2574881)
Target
See all TNF alpha AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 77-233
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Specificity
- The antibody is a rabbit polyclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.
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Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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Immunogen
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Recombinant TNFa (Val77-Leu233) expressed in E. coli.
Type of Immunogen: Recombinant protein -
Isotype
- IgG
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Application Notes
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Approved: WB (1:100 - 1:400)
Usage: ELISA 1:100-1:5000, ICC 1:50-500, WB 1:50-400, -
Comment
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Target Species of Antibody: Monkey
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- PBS, pH 7.4, 0.02 % sodium azide, 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 4°C for frequent use. Aliquot and store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
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Expiry Date
- 12 months
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- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Alternative Name
- TNF alpha
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Background
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Name/Gene ID: TNF
Family: TNF
Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2 -
Gene ID
- 7124
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Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
Target
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