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GFP antibody
The Goat Polyclonal anti-GFP antibody has been validated for WB, IF, IHC (fro) and IHC (p). It is suitable to detect GFP in samples from Aequorea victoria.
Quick Overview for GFP antibody (ABIN2603641)
Target
See all GFP Antibodies
GFP
(Green Fluorescent Protein (GFP))
Reactivity
All reactivities for GFP antibodies
Aequorea victoria
Host
All hosts for GFP antibodies
Goat
Clonality
All clonalities for GFP antibodies
Polyclonal
Conjugate
All conjugates for GFP antibodies
This GFP antibody is un-conjugated
Application
All applications for GFP antibodies
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Product Details anti-GFP Antibody
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Specificity
In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognise mCherry fluorescent protein.
Purification
Immunoaffinity purified
Immunogen
Purified recombinant peptide produced in E. coli. Type of Immunogen: Recombinant protein
Isotype
IgG
Alternatives
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Application Details
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Application Notes
Approved: IF (1:50 - 1:1000), IHC-Fr (1:50 - 1:1000), IHC-P (1:50 - 1:1000), WB (1:500 - 1:2000)
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
Lot specific
Buffer
PBS, 20 % glycerol, 0.05 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Details for GFP
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Target
GFP
(Green Fluorescent Protein (GFP))
Alternative Name
GFP
Background
Name/Gene ID: GFP Synonyms: GFP
UniProt
P42212
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