Integrin beta 2 antibody (AA 86-99)
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- Target See all Integrin beta 2 (ITGB2) Antibodies
- Integrin beta 2 (ITGB2)
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Binding Specificity
- AA 86-99
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Integrin beta 2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Purpose
- ITGB2 / LFA-1 (aa86-99)
- Sequence
- ETQEDHNGGQ KQLS
- Specificity
- Reported variants represent identical protein: NP_001120963.1, NP_000202.2.
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Isotype
- IgG
- Top Product
- Discover our top product ITGB2 Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.
Western Blot: Approx 90 kDa band observed in lysates of cell lines K562 and U937 (calculated MW of 84.8 kDa according to NP_00202.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:128000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Integrin beta 2 (ITGB2)
- Alternative Name
- ITGB2 (ITGB2 Products)
- Synonyms
- CD18 antibody, LAD antibody, LCAMB antibody, LFA-1 antibody, MAC-1 antibody, MF17 antibody, MFI7 antibody, 2E6 antibody, AI528527 antibody, Cd18 antibody, Lfa1 antibody, ITGB2 antibody, itgb2 antibody, itgb2-a antibody, itgb2-b antibody, integrin subunit beta 2 antibody, integrin beta 2 antibody, integrin subunit beta 2 S homeolog antibody, ITGB2 antibody, Itgb2 antibody, itgb2.S antibody
- Background
- ITGB2, integrin, beta 2 (complement component 3 receptor 3 and 4 subunit), CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7, cell surface adhesion glycoprotein LFA-1/CR3/P150,959 beta subunit precursor), cell surface adhesion glycoproteins LFA-1/CR3/p150,95 sub
- Gene ID
- 3689
- NCBI Accession
- NP_000202
- Pathways
- NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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