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Robo3.2 (AA 1303-1316) antibody

Verified Reactivity: Human ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN2613474
  • Target
    Robo3.2
    Binding Specificity
    AA 1303-1316
    Reactivity
    Human
    Host
    • 1
    Goat
    Clonality
    • 1
    Polyclonal
    Conjugate
    • 1
    Un-conjugated
    Application
    ELISA, Immunohistochemistry (IHC)
    Purpose
    Robo3.2 (mouse aa1303-1316)
    Sequence
    KESQGRGRGL EACR
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Pig, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    KESQGRGRGLEACR
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5 μg/mL.

    Western Blot: Preliminary experiments gave bands at approx 85 kDa in Mouse fetal Brain lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Robo3.2
    Background
    Robo3, roundabout homolog 3 (Drosophila), Rbig1, Rig-1, Rig1, Robo3a, Robo3b, retinoblastoma inhibiting gene 1, retinoblastoma-inhibiting gene 1 protein, roundabout homolog 3
    Gene ID
    19649
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