ACTN1 antibody (AA 596-609)

Catalog No. ABIN2614226

Product Details anti-ACTN1 Antibody

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Binding Specificity: AA 596-609

Reactivity: Human, Mouse, Rat, Monkey, Rabbit, Cow, Guinea Pig, Horse, Pig, Bat, Sheep

Host: Goat

Clonality: Polyclonal

Conjugate: This ACTN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human ACTN1. This antibody is expected to recognize all reported isoforms (NP_001123476.1, NP_001093.1, NP_001123477.1).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-TPQEINGKWDHVRQ from an internal region of human ACTN1 (NP_001123476.1, NP_001093.1, NP_001123477.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Mouse, Rat, Ferret, Sheep, Bovine, Bat, Panda, Rabbit, Horse, Pig, Guinea pig, Platypus (100%), Elephant, Turkey, Chicken, Xenopus (93%), Zebra finch, Lizard (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:128000), WB (0.3 - 1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 100kD band observed in Human Spleen lysates (calculated MW of 103kD according to NP_001093.1). Recommended concentration: 0.3-1 μg/mL. An additional band of unknown identity was also consistently observed at 75kD. This band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ACTN1

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Alternative Name: ACTN1 (ACTN1 Products)

Synonyms: ACTN1 antibody, actinin antibody, BDPLT15 antibody, 3110023F10Rik antibody, Actn1a antibody, actinin alpha 1 antibody, actinin, alpha 1 antibody, actinin alpha 1 L homeolog antibody, ACTN1 antibody, actn1 antibody, Actn1 antibody, actn1.L antibody

Background: Name/Gene ID: ACTN1

Synonyms: ACTN1, Alpha-actinin-1, Actinin 1 smooth muscle, Actinin, alpha 1, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ASMA

Gene ID: 87

NCBI Accession: NP_001123476, NP_001093, NP_001123477

Pathways: Cell-Cell Junction Organization