IGJ antibody (Biotin)

Catalog No. ABIN2624384

Product Details anti-IGJ Antibody (Biotin)

Target: IGJ (Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IGJ antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Dot Blot (DB)

Specificity: Human J chain of dimeric IgA.

Purification: Purified IgG

Immunogen: Human J chain is a polypeptide folded within the structure of the polymeric immunoglobulin, resulting in a very limited exposure of J chain antigenic determinants. The antiserum is raised against the isolated and purified J chain. J chains isolated from polymeric IgA and IgM are identical by criteria of composition, peptide maps and antigenicity. Human J chain is distinct from all other chain components of polymeric IgA and IgM. It has a unique primary structure as shown by sequence analyses. Fr

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: DB, ELISA, ICC, IHC, IHC-P

Usage: In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), this immunoconjugate shows a single precipitation reaction with totally reduced and alkylated polyclonal and monoclonal polymeric IgA, secretory IgA and IgM. A reaction of complete identity is obtained with precipitated highly purified J chain and with the single of precipitation obtained with normal human serum after two hours incubation with 9 M urea and 0.2 M mercaptoethanol at  pH 8.6. In a competitive radioimmunoassay no inhibition is obtained with monomeric IgA, polyclonal IgG, reduced and alkylated alpha, mu, gamma, kappa and lambda light chains (less than 1 percent over background). In immunocytochemical and immunohistochemical techniques for the detection of human J chain at the cellular and subcellular level in appropriately treated cell and tissue substrates. as detection reagent in nonisotopic methodology and solid phase immunochemistry (e. g. ELISA, Western blotting). As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:200 and 1:10000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IGJ

Target: IGJ (Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ))

Alternative Name: JCHAIN / Ig J Chain (IGJ Products)

Synonyms: IGJ antibody, DKFZp469K0627 antibody, IGCJ antibody, JCH antibody, 9530090F24Rik antibody, AI323815 antibody, Jch antibody, joining chain of multimeric IgA and IgM antibody, immunoglobulin joining chain antibody, Jchain antibody, JCHAIN antibody

Background: Name/Gene ID: JCHAIN

Synonyms: JCHAIN, IGCJ, Immunoglobulin J chain, IGJ, IgJ chain, JCH

Gene ID: 3512