IGJ antibody (FITC)
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- Target See all IGJ Antibodies
- IGJ (Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ))
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This IGJ antibody is conjugated to FITC
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Application
- Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- Human J chain of dimeric IgA.
- Purification
- Purified IgG
- Immunogen
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Human J chain is a polypeptide folded within the structure of the polymeric immunoglobulin, resulting in a very limited exposure of J chain antigenic determinants. The antiserum is raised against the isolated and purified J chain. J chains isolated from polymeric IgA and IgM are identical by criteria of composition, peptide maps and antigenicity. Human J chain is distinct from all other chain components of polymeric IgA and IgM. It has a unique primary structure as shown by sequence analyses. Fr
Type of Immunogen: Purified protein - Top Product
- Discover our top product IGJ Primary Antibody
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- Application Notes
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Approved: ELISA, ICC, IF, IHC, IHC-Fr
Usage: In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), this immunoconjugate shows a single precipitation reaction with totally reduced and alkylated polyclonal and monoclonal polymeric IgA, secretory IgA and IgM. A reaction of complete identity is obtained with precipitated highly purified J chain and with the single of precipitation obtained with normal human serum after two hours incubation with 9 M urea and 0.2 M mercaptoethanol at pH 8.6. In a competitive radioimmunoassay no inhibition is obtained with monomeric IgA, polyclonal IgG, reduced and alkylated alpha, mu, gamma, kappa and lambda light chains (less than 1 percent over background). In immunocytochemical and immunohistochemical techniques for the detection of human J chain at the cellular and subcellular level in appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:50. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2. No preservative added.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- IGJ (Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ))
- Alternative Name
- JCHAIN / Ig J Chain (IGJ Products)
- Synonyms
- IGJ antibody, DKFZp469K0627 antibody, IGCJ antibody, JCH antibody, 9530090F24Rik antibody, AI323815 antibody, Jch antibody, joining chain of multimeric IgA and IgM antibody, immunoglobulin joining chain antibody, Jchain antibody, JCHAIN antibody
- Background
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Name/Gene ID: JCHAIN
Synonyms: JCHAIN, IGCJ, Immunoglobulin J chain, IGJ, IgJ chain, JCH - Gene ID
- 3512
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