Immunohistochemistry. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
5.0mg/mL (OD280nm, E0.1% = 1.4)
Buffer
10 mM Phosphate, pH 7.2 containing 150 mM Sodium chloride and 0,09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one week or (in aliquots) at -40 °C for longer. If aliquoted for long term storage, fill volume should be equal to or greater than 50% of thenominal fill volume of the vial used. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
This is a major plasma protein produced by the yolk sac and the liver during fetal life. alpha Fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha Fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha Fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. alpha Fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha Fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP