This antibody reacts with free cardiac troponin I (cTnI). Reacts weakly with cTnI forming complexes with other troponin components. Not affected by phosphorylation. Recognizes an epitope located between amino acid residues 148 and 158 of cTnI. No cross-reactivity with skeletal muscle troponin I.
Immunoassay. Immunoprecipitation. Immunohistochemistry. Immunoaffinity purification. Western blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
3.1mg/mL (Sigma protein assay kit)
Buffer
PBS, pH 7.4 containing 0,09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! Shelf life: one year from despatch.
Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.Synonyms: TNNC1, TNNI3