CDK4 antibody

Catalog No. ABIN264334

This Mouse Monoclonal antibody specifically detects CDK4 in WB, IF and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CDK4 antibody (ABIN264334)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: DCS-31-2

Product Details anti-CDK4 Antibody

Characteristics: Synonyms: PSK-J3, Cell division protein kinase 4, Cyclin-dependent kinase 4

Purification: Purified

Immunogen: Recombinant cdk 4 protein recognizing a 34kD protein.

Isotype: IgG1

Application Details

Application Notes: Western Blot. Immunoprecipitation (unbound cdk4 only). Immunofluorescense.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.08 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: CDK4

Background: Cdk4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk4 is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1/S phase, which is controlled by the regulatory subunits D type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). The mutations in this gene as well as its related proteins including D type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Two alternatively spliced variants, and multiple polyadenylation sites of this gene have been reported.Synonyms: Cell division protein kinase 4, Cyclin-dependent kinase 4, PSK-J3

Gene ID: 1019

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size