Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with asynthetic peptide derived from the Cterminus of the human CYP450 4F11 protein andmouse myeloma Ag8563 cells.
ELISA. Western Blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections: 1 - 5 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
Phosphate buffered saline with 0.08 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
CYP4F11
(Cytochrome P450, Family 4, Subfamily F, Polypeptide 11 (CYP4F11))
CYPIVF11 antibody, cytochrome P450, family 4, subfamily F, polypeptide 11 antibody, cytochrome P450 family 4 subfamily F member 11 antibody, CYP4F11 antibody
Background
The cytochrome P450 proteins (CYPs) are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. P450 enzymes are classified into subfamilies based on their sequence similarities. CYP4F11 is an isoform of the cytochrome P450 4F protein and is expressed mainly in human liver, followed by kidney, heart, and skeletal muscle. CYP4F11 contains 524 amino acid residues that share 80.0, 82.3 and 79.2 % identity to CYP4F2, CYP4F3, and CYP4F8 amino acid sequences, respectively. CYP4F11 has catalytic properties towards endogenous eicosanoids as well as some clinically relevant drugs, and is able to metabolize large molecules such as erythromycin.Synonyms: CYPIVF11, Cytochrome P450 4F11