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- Target
- Histone 1
- Reactivity
- Cow
- Host
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Sheep
- Clonality
- Polyclonal
- Application
- Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
- Cross-Reactivity (Details)
- Histone-H-1 100%, No reaction with Histone-H-2b, Histone-H-3 or Histone-H-4Very low crossreaction with Histone-H-core
- Purification
- purified
- Immunogen
- Histone-1 antibody was raised in sheep using Intact calf histone H1 complexed with RNA. as the immunogen.
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- Application Notes
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IF: 1:100, IP: 1:20, WB: 1:300
Optimal conditions should be determined by the investigator. - Restrictions
- For Research Use only
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- Concentration
- Lot specific
- Buffer
- Protein G purified and supplied in PBS, pH 7.4 .
- Handling Advice
-
Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - Storage
- -20 °C/-80 °C
- Storage Comment
- Aliquot and store at -20 °C for short term storage, -70 °C for long-term storage.
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- Target
- Histone 1
- Background
- Histones are highly alkaline proteins found in eukaryotic cell nuclei, which package and order the DNA into structural units called nucleosomes. They are the chief protein components of chromatin, acting as spools around which DNA winds, and play a role in gene regulation.
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