CEBPB
Reactivity: Human, Mouse, Rat
WB, ELISA, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
CCAAT-enhancer binding proteins (C/EBP) are basic region/leucine zipper (bZIP) transcription factors selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP β is a member of the C/EBP transcription factor family. The C/EBP β gene encodes several isoforms, which have truncated transcription activation domains by the alternative translational initiation at multipe AUG start sites. Initiation of translation at the in-frame AUGs forms four C/EBP β isoforms. C/EBP β is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein(LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C/EBP β contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C/EBP β gene that has direct implication for acute phase response in liver cells.The 35 kDa C/EBP β form requires phosphorylation for its DNA binding ability, and increased binding of C/EBP β isoforms during acute-phase reaction occurs through its upregulation and structural modification.Synonyms: C/EBP beta, CCAAT/enhancer-binding protein beta, LAP, Liver activator protein, NF-IL6, Nuclear factor NF-IL6, TCF5, Transcription factor 5