POLB antibody

Catalog No. ABIN265385

The Rabbit Polyclonal anti-POLB antibody is suitable to detect POLB in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for POLB antibody (ABIN265385)

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-POLB Antibody

Specificity: This antibody detects endogenous levels of DNA polymerase beta / DPOLB protein (region surrounding Gln324).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Alternative Name: DNA Polymerase beta

Background: DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases. DNA pol α, β, δ and ε are involved in DNA replication and repair. DNA pol δ and DNA pol ε are multisubunit enzymes, with DNA pol δ consisting of two subunits-p125, which interacts with the sliding DNA clamp protein PCNA, and p50. The nuclear-encoded DNA pol γ is the only DNA polymerase required for the replication of the mitochondrial DNA. DNA pol ζ is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis. DNA pol θ is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks.Synonyms: POLB

Molecular Weight: approx. 38 kDa

Gene ID: 5423

NCBI Accession: NP_002681

UniProt: P06746

Pathways: DNA Damage Repair