GFP antibody (Alexa Fluor 488)

Catalog No. ABIN2657082

This Rat Monoclonal antibody specifically detects GFP in IF and FACS. It exhibits reactivity toward Aequorea victoria.

Quick Overview for GFP antibody (Alexa Fluor 488) (ABIN2657082)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rat

Clonality: Monoclonal

Conjugate: This GFP antibody is conjugated to Alexa Fluor 488

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: FM264G

Product Details anti-GFP Antibody (Alexa Fluor 488)

Purification: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Green fluorescent protein (GFP) was originally identified as a protein involved in bioluminescence, which is from the jellyfish Aequorea Victoria. It is widely used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces without the need for exogenous substrates or cofactors, or other intrinsic or extrinsic proteins, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Purified GFP is a 27 kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.