SIRT1 antibody (AA 581-630)

Catalog No. ABIN2668278

This Mouse Monoclonal antibody specifically detects SIRT1 in WB, IP, ICC and IF. It exhibits reactivity toward Human. It has been mentioned in 2+ publications

Quick Overview for SIRT1 antibody (AA 581-630) (ABIN2668278)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 2G1-F7

Product Details anti-SIRT1 Antibody

Binding Specificity: AA 581-630

Purpose: SIRT1 antibody (mAb) (Clone 2G1/F7)

Purification: Protein G Chromatography

Immunogen: This SIRT1 antibody was raised against a synthetic peptide corresponding to amino acids 581-630 of human SIRT1.

Isotype: IgG1

Application Details

Application Notes: WB: 0.5 - 2.0 μg/mL dilution The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: 10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30 % glycerol, 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-SIRT1 antibody (ABIN2668278)

Rajendran, Kidane, Yu, Dashwood, Bisson, Löhr, Ho, Williams, Dashwood: "HDAC turnover, CtIP acetylation and dysregulated DNA damage signaling in colon cancer cells treated with sulforaphane and related dietary isothiocyanates." in: Epigenetics, Vol. 8, Issue 6, pp. 612-23, (2013) (PubMed).

Vaquero, Scher, Lee, Erdjument-Bromage, Tempst, Reinberg: "Human SirT1 interacts with histone H1 and promotes formation of facultative heterochromatin." in: Molecular cell, Vol. 16, Issue 1, pp. 93-105, (2004) (PubMed).

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Background: SIRT1 (Sirtuin 1, SIR2L1) is a member of the Sirtuin family of proteins related to the yeast Sir2 protein. Like Sir2 and other Sirtuins, SIRT1 is an NAD-dependent histone and protein deacetylase. SIRT1 catalyzes the removal of acetyl groups from a variety of protein substrates (NBS1, p53, Ku70, etc.) as well as from Histone H4 Lys16. SIRT1 is involved in the regulation of a number of cellular processes, including transcription, metabolism, DNA repair and aging, and increased SIRT1 is associated with extended lifespan of C. elegans.

Molecular Weight: 120 kDa

Gene ID: 23411

NCBI Accession: NP_036370

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling