Rpb1 CTD antibody (Tyr1)

Catalog No. ABIN2668519

There are 4+ publications for this product available. The Rat Monoclonal anti-Rpb1 CTD antibody is suitable to detect Rpb1 CTD in samples from Human. It has been validated for WB, ChIP and IP.

Quick Overview for Rpb1 CTD antibody (Tyr1) (ABIN2668519)

Target: Rpb1 CTD (RNA Polymerase II CTD)

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This Rpb1 CTD antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP)

Clone: 3D12

Product Details anti-Rpb1 CTD Antibody

Binding Specificity: Tyr1

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Rpb1 CTD antibody (ABIN2668519)

Harlen, Trotta, Smith, Mosaheb, Fuchs, Churchman: "Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue." in: Cell reports, Vol. 15, Issue 10, pp. 2147-2158, (2016) (PubMed).

Laitem, Zaborowska, Isa, Kufs, Dienstbier, Murphy: "CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes." in: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 396-403, (2015) (PubMed).

Zaborowska, Baumli, Laitem, OReilly, Thomas, OHare, Murphy: "Herpes Simplex Virus 1 (HSV-1) ICP22 protein directly interacts with cyclin-dependent kinase (CDK)9 to inhibit RNA polymerase II transcription elongation." in: PLoS ONE, Vol. 9, Issue 9, pp. e107654, (2014) (PubMed).

Mayer, Heidemann, Lidschreiber, Schreieck, Sun, Hintermair, Kremmer, Eick, Cramer: "CTD tyrosine phosphorylation impairs termination factor recruitment to RNA polymerase II." in: Science (New York, N.Y.), Vol. 336, Issue 6089, pp. 1723-5, (2012) (PubMed).

Target Details for Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Alternative Name: RNA Pol II CTD

Molecular Weight: 210 kDa

Gene ID: 5430