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Rpb1 CTD antibody (pSer2)

Reactivity: Human ICC, IF, WB Host: Rat Monoclonal 3E7C7 unconjugated
Catalog No. ABIN6972624
$605.00
Plus shipping costs $45.00
100 μg
Shipping to: United States
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  • Target See all Rpb1 CTD products
    Rpb1 CTD (RNA Polymerase II CTD)
    Binding Specificity
    • 17
    • 10
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    pSer2
    Reactivity
    • 38
    • 21
    • 16
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 24
    • 10
    • 4
    Rat
    Clonality
    • 24
    • 14
    Monoclonal
    Conjugate
    • 24
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This Rpb1 CTD antibody is un-conjugated
    Application
    • 24
    • 18
    • 15
    • 11
    • 7
    • 4
    • 4
    • 3
    • 2
    • 1
    Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
    Characteristics
    RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation. RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E7C7) was raised in a Rat host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples.
    Purification
    Protein G Chromatography
    Immunogen
    This antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 2.
    Clone
    3E7C7
    Isotype
    IgG2a
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Buffer
    Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
  • Target
    Rpb1 CTD (RNA Polymerase II CTD)
    Alternative Name
    RNA pol II CTD (Rpb1 CTD Products)
    Synonyms
    5, 8WG16, CG1554, CTD, Dmel\\CG1554, H5, II, L5, POL, Pol II, Pol II CTD, Pol II Ser5P, Pol II0[ser2], Pol II0[ser5], Pol II[ser2], Pol IIa, Pol IIo, Pol IIo[Ser2], Pol IIo[ser2], Pol IIo[ser5], Pol-IIa, PolII, PolIIo, RNA Pol II, RNA Pol II CTD, RNA PolI 215, RNA PolII, RNA pol II, RNA pol IIo, RNA polII, RNAP, RNAP II, RNAP II LS, RNAPII, RNAPII0, RNApol2, RNApolII, RPB1, RPII215, RpII, Rpb1, Rpll215, Ubl, dRPB1, dRpb1, l(1)10Ca, l(1)DC912, l(1)DF912, l(1)G0040, l(1)L5, pol II, polII, RNA polymerase II 215kD subunit, RpII215
    Background
    RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation.
    Molecular Weight
    240 kDa
    NCBI Accession
    NP_000928
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