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Rpb1 CTD antibody (pSer5)

Reactivity: Human WB, IF, ICC Host: Rat Monoclonal 1H4B6 unconjugated
Catalog No. ABIN6972638
  • Target See all Rpb1 CTD products
    Rpb1 CTD (RNA Polymerase II CTD)
    Binding Specificity
    • 14
    • 11
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    pSer5
    Reactivity
    • 36
    • 20
    • 16
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 22
    • 10
    • 4
    Rat
    Clonality
    • 22
    • 14
    Monoclonal
    Conjugate
    • 22
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This Rpb1 CTD antibody is un-conjugated
    Application
    • 22
    • 16
    • 13
    • 10
    • 7
    • 4
    • 4
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Characteristics
    RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at Serine 5 and Serine 2. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription. RNA pol II CTD phospho Ser5 antibody (mAb) (Clone 1H4B6) was raised in a Rat host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples.
    Purification
    Protein G Chromatography
    Immunogen
    This antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 5.
    Clone
    1H4B6
    Isotype
    IgG2b
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Buffer
    Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
  • Target
    Rpb1 CTD (RNA Polymerase II CTD)
    Alternative Name
    RNA pol II CTD (Rpb1 CTD Products)
    Synonyms
    5 antibody, 8WG16 antibody, CG1554 antibody, CTD antibody, Dmel\\CG1554 antibody, H5 antibody, II antibody, L5 antibody, POL antibody, Pol II antibody, Pol II CTD antibody, Pol II Ser5P antibody, Pol II0[ser2] antibody, Pol II0[ser5] antibody, Pol II[ser2] antibody, Pol IIa antibody, Pol IIo antibody, Pol IIo[Ser2] antibody, Pol IIo[ser2] antibody, Pol IIo[ser5] antibody, Pol-IIa antibody, PolII antibody, PolIIo antibody, RNA Pol II antibody, RNA Pol II CTD antibody, RNA PolI 215 antibody, RNA PolII antibody, RNA pol II antibody, RNA pol IIo antibody, RNA polII antibody, RNAP antibody, RNAP II antibody, RNAP II LS antibody, RNAPII antibody, RNAPII0 antibody, RNApol2 antibody, RNApolII antibody, RPB1 antibody, RPII215 antibody, RpII antibody, Rpb1 antibody, Rpll215 antibody, Ubl antibody, dRPB1 antibody, dRpb1 antibody, l(1)10Ca antibody, l(1)DC912 antibody, l(1)DF912 antibody, l(1)G0040 antibody, l(1)L5 antibody, pol II antibody, polII antibody, RNA polymerase II 215kD subunit antibody, RpII215 antibody
    Molecular Weight
    240 kDa
    NCBI Accession
    NP_000928
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