Donkey anti-Sheep IgG (Heavy & Light Chain) Antibody (PE) - Preadsorbed

Catalog No. ABIN2669896

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Sheep

Host: Donkey

Clonality: Polyclonal

Conjugate: PE

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Sheep IgG and Sheep Serum.

Cross-Reactivity: Sheep (Ovine)

Characteristics: Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated antibody generated in donkey detects specifically sheep IgG. This secondary phycoerythrin conjugated antibody anti-Sheep is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.
Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated antibody generated in donkey detects specifically sheep IgG. This secondary phycoerythrin conjugated antibody anti-Sheep is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Anti-Sheep IgG was produced by repeated immunization with sheep IgG whole molecule in donkey.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Antibody Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. Optimal titers for other applications should be determined by the researcher.

Flow Cytometry Dilution: 1:100 - 1:250

IF Microscopy Dilution: 1:100 - 1:250

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light.

Storage: RT,4 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Donkey F(ab')2 Anti-Sheep IgG Antibody Phycoerythrin Conjugation, Donkey F(ab')2 Anti-Sheep IgG PE Conjugated Antibody

Background: Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated antibody generated in donkey detects specifically sheep IgG. This secondary phycoerythrin conjugated antibody anti-Sheep is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.