IFNGR1 antibody (Biotin)

Catalog No. ABIN2688898

Product Details anti-IFNGR1 Antibody (Biotin)

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IFNGR1 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The GR20 antibody reacts with CD119, the α chain of the interferon γ (IFN-γ) receptor. This receptor is expressed by mouse leukocytes, endothelial, and epithelial cells, but not by mature erythrocytes. GR20 mAb recognizes an epitope (susceptible to certain fixatives) in the ligand-binding site of the receptor. It can block the binding of mouse IFN-γ, therefore, it can inhibit IFN-γ .mediated effects including the activation of mouse macrophages for tumor cell killing. IFN-γ has been reported to induce the expression of Ly-6A/E antigen on cell lines and normal B lymphocytes, and the GR20 antibody is capable of inhibiting the IFN-γ.induced expression of Ly-6A/E on the mouse B lymphoma line A20. The binding of the GR20 antibody to CD119 is inhibited by IFN-γ. This antibody reportedly does not have any agonist effect.

BD Pharmingen™ Biotin Rat Anti-Mouse CD119 - Biotin - Clone GR20 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: BALB/c mouse monomyelocytic cell line WEHI-3

Clone: GR20

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-IFNGR1 antibody (Biotin) (ABIN2688898)

LeClaire, Basu, Pinson, Redick, Hunt, Zavodny, Pace, Russell: "Characterization and use of monoclonal and polyclonal antibodies against the mouse interferon-gamma receptor." in: Journal of leukocyte biology, Vol. 51, Issue 5, pp. 507-16, (1992) (PubMed).

Cofano, Moore, Tanaka, Yuhki, Landolfo, Appella: "Affinity purification, peptide analysis, and cDNA sequence of the mouse interferon gamma receptor." in: The Journal of biological chemistry, Vol. 265, Issue 7, pp. 4064-71, (1990) (PubMed).

Codias, Malek: "Regulation of B lymphocyte responses to IL-4 and IFN-gamma by activation through Ly-6A/E molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 6, pp. 2197-204, (1990) (PubMed).

Basu, Pace, Pinson, Hayes, Trotta, Russell: "Purification and partial characterization of a receptor protein for mouse interferon gamma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 17, pp. 6282-6, (1988) (PubMed).

Target Details for IFNGR1

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Alternative Name: CD119 (IFNGR1 Products)

Synonyms: CD119 antibody, IFNGR antibody, IFN-gammaR antibody, Ifgr antibody, Ifngr antibody, Nktar antibody, IFNGR1 antibody, MGC129098 antibody, ifngr1 antibody, MGC194858 antibody, MGC194873 antibody, zgc:194858 antibody, cd119 antibody, ifngr antibody, DKFZp469K1232 antibody, interferon gamma receptor 1 antibody, cytokine receptor family member B17 antibody, IFNGR1 antibody, Ifngr1 antibody, crfb17 antibody, ifngr1 antibody

Background: Synonyms: IFN-γ receptor α chain

Pathways: Interferon-gamma Pathway