CD11c antibody

Catalog No. ABIN2688920

Product Details anti-CD11c Antibody

Target: CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CD11c antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Brand: BD Pharmingen™

Characteristics: The HL3 monoclonal antibody specifically binds to the integrin αx chain of gp150, 95 (CD11c/CD18) which is expressed on dendritic cells and CD4- CD8+ intestinal intraepithelial lymphocytes (IEL) and is upregulated on IEL and lymph-node T cells following in vivo activation. CD11c is also found on human NK cells. Although its expression on mouse NK cells is not published, we have detected CD11c on mouse splenic NK cells. Cells of the monocyte/macrophage lineage have been reported to express low levels of CD11c. CD11c plays a role in binding of iC3b. Expression of CD11c on spleen dendritic cells. The frozen section of normal mouse spleen was stained with HL3 mAb. Note the brown labeling of irregular-shaped cells in the red pulp surrounding lymphoid follicles.

BD Pharmingen™ Purified Hamster Anti-Mouse CD11c - Purified - Clone HL3 - Isotype Armenian Hamster IgG1, λ2 - Reactivity Ms - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: C57BL/6 Mouse Intestinal Intraepithelial Lymphocytes

Clone: HL3

Isotype: IgG1 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 125 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD11c antibody (ABIN2688920)

Fagarasan, Muramatsu, Suzuki, Nagaoka, Hiai, Honjo: "Critical roles of activation-induced cytidine deaminase in the homeostasis of gut flora." in: Science (New York, N.Y.), Vol. 298, Issue 5597, pp. 1424-7, (2002) (PubMed).

Huleatt, Lefrançois: "Antigen-driven induction of CD11c on intestinal intraepithelial lymphocytes and CD8+ T cells in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 11, pp. 5684-93, (1995) (PubMed).

Larson, Springer: "Structure and function of leukocyte integrins." in: Immunological reviews, Vol. 114, pp. 181-217, (1990) (PubMed).

Target Details for CD11c

Target: CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))

Alternative Name: CD11c (ITGAX Products)

Synonyms: CD11C antibody, SLEB6 antibody, RGD1561123 antibody, AI449405 antibody, Cd11c antibody, Cr4 antibody, N418 antibody, integrin subunit alpha X antibody, integrin alpha X antibody, ITGAX antibody, Itgax antibody

Background: Synonyms: Cd11c, Itgax, Integrin alpha-X, Integrin αX, Cr4, Complement receptor 4

Pathways: Complement System, Activated T Cell Proliferation, Integrin Complex