Insulin Receptor antibody

Catalog No. ABIN2689096

Cited in 3+ publications. The Mouse Monoclonal anti-Insulin Receptor antibody (Clone 3B6-IR) (ABIN2689096) specifically detects Insulin Receptor in FACS. The antibody is reactive with Human samples.

Quick Overview for Insulin Receptor antibody (ABIN2689096)

Target: Insulin Receptor (INSR)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 3B6-IR

Product Details anti-Insulin Receptor Antibody

Characteristics: Reacts with insulin receptor (IR), a glycoprotein composed of two extracellular α-subunits (130 kDa) and two transmembrane β-subunits (95 kDa). It is expressed on human hematopoietic and non-hematopoietic cells, but unlike the insulin-like growth factor receptor (IGF-IR), which is ubiquitous, the insulin receptor is restricted to major target tissues of insulin action. Upon binding insulin, the IR subunits form a heterotetramer of two α and two β subunits resulting in autophosphorylation and activation of the tyrosine kinase activity of the receptor. This ligand-receptor interaction is important in regulating cell metabolism and growth. 3B6/IR monoclonal antibody reacts similarly to anti-human IR α, clone 47-9, an α-subunit antibody. Profile of peripheral blood monocytes analyzed by flow cytometry. Second step staining with Cat. No. 555988.

BD Pharmingen™ Purified Mouse Anti-Human CD220 - Purified - Clone 3B6/IR - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Insulin Receptor antibody (ABIN2689096)

Prigent, Stanley, Siddle: "Identification of epitopes on the human insulin receptor reacting with rabbit polyclonal antisera and mouse monoclonal antibodies." in: The Journal of biological chemistry, Vol. 265, Issue 17, pp. 9970-7, (1990) (PubMed).

Soos, OBrien, Brindle, Stigter, Okamoto, Whittaker, Siddle: "Monoclonal antibodies to the insulin receptor mimic metabolic effects of insulin but do not stimulate receptor autophosphorylation in transfected NIH 3T3 fibroblasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 14, pp. 5217-21, (1989) (PubMed).

Soos, Siddle, Baron, Heward, Luzio, Bellatin, Lennox: "Monoclonal antibodies reacting with multiple epitopes on the human insulin receptor." in: The Biochemical journal, Vol. 235, Issue 1, pp. 199-208, (1986) (PubMed).

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: CD220

Background: Synonyms: Insulin Receptor

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity