Selectin E/CD62e antibody (Biotin)
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- Target See all Selectin E/CD62e (SELE) Antibodies
- Selectin E/CD62e (SELE) (Selectin E (SELE))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Selectin E/CD62e antibody is conjugated to Biotin
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Application
- Flow Cytometry (FACS)
- Brand
- BD Pharmingen™
- Characteristics
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Reacts with the 97-115 kDa cell surface glycoprotein, E-selectin, also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1). E-selectin is thought to be involved in the initial interaction of neutrophils with inflammed endothelium. CD62E is expressed on cytokine-stimulated endothelium, but in minimal amounts on unstimulated endothelium. The adhesion between endothelial E-selectin molecules and a carbohydrate ligand on neutrophils is inhibited by the mAb 68-5H11. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of TNFa-stimulated HUVEC cells analyzed on a FACScan
BD Pharmingen™ Biotin Mouse Anti-Human CD62E - Biotin - Clone 68-5H11 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Clone
- 68
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product SELE Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex." in: Science (New York, N.Y.), Vol. 250, Issue 4984, pp. 1130-2, (1991) (PubMed).
: "Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins." in: Science (New York, N.Y.), Vol. 243, Issue 4895, pp. 1160-5, (1989) (PubMed).
: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).
: "
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ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex." in: Science (New York, N.Y.), Vol. 250, Issue 4984, pp. 1130-2, (1991) (PubMed).
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- Target
- Selectin E/CD62e (SELE) (Selectin E (SELE))
- Alternative Name
- CD62E (SELE Products)
- Synonyms
- SELE antibody, CD62E antibody, ELAM antibody, ELAM1 antibody, ESEL antibody, LECAM2 antibody, E-selectin antibody, Elam antibody, selectin E antibody, selectin, endothelial cell antibody, SELE antibody, Sele antibody
- Background
- Synonyms: E-Selectin
- Pathways
- Thromboxane A2 Receptor Signaling
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