CD86 antibody

Catalog No. ABIN2689419

Product Details anti-CD86 Antibody

Target: CD86

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD86 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: IT2.2 recognizes a 75 kDa cell surface protein, CD86 (B70/B7-2), expressed primarily on monocytes and activated B cells including lymphoid cells in germinal centers and Epstein-Barr virus transformed B-cell lines. CD86 is the second ligand for CD28 and CTLA-4 and may play an important role in costimulation of T cells in primary immune response. Competitive binding assays demonstrate that, while both IT2.2 and FUN-1 (anti-CD86, Cat. No. 555665) recognize the same molecule, they react with different epitopes. IT2.2 blocks costimulation activity of CD86 in functional studies and blocks binding of human CTLA-4-Ig fusion protein to B70 gene-transfected cells. Profile of Daudi cell line analyzed by flow cytometry

BD Pharmingen™ Purified Mouse Anti-Human CD86 - Purified - Clone IT2.2 - Isotype Mouse IgG2b, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: IT2-2

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD86 antibody (ABIN2689419)

Engel, Gribben, Freeman, Zhou, Nozawa, Abe, Nadler, Wakasa, Tedder: "The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen." in: Blood, Vol. 84, Issue 5, pp. 1402-7, (1994) (PubMed).

Azuma, Ito, Yagita, Okumura, Phillips, Lanier, Somoza: "B70 antigen is a second ligand for CTLA-4 and CD28." in: Nature, Vol. 366, Issue 6450, pp. 76-9, (1993) (PubMed).

Nozawa, Wachi, Tominaga, Abe, Wakasa: "A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells." in: The Journal of pathology, Vol. 169, Issue 3, pp. 309-15, (1993) (PubMed).

Target Details for CD86

Target: CD86

Alternative Name: CD86 (CD86 Products)

Synonyms: B7-2 antibody, B7.2 antibody, B70 antibody, CD28LG2 antibody, LAB72 antibody, B7 antibody, CLS1 antibody, Cd28l2 antibody, ETC-1 antibody, Ly-58 antibody, Ly58 antibody, MB7 antibody, MB7-2 antibody, TS/A-2 antibody, CD86 molecule antibody, CD86 antigen antibody, integrin subunit alpha 2b antibody, CD86 antibody, Cd86 antibody, Itga2b antibody

Background: Synonyms: B70/B7-2

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation