EPH Receptor B2 antibody

Catalog No. ABIN2689499

Product Details anti-EPH Receptor B2 Antibody

Target: EPH Receptor B2 (EPHB2)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPH Receptor B2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: The 2H9 monoclonal antibody specifically binds to the Ephrin Type-B Receptor 2 (EphB2). EphB2 is a type I transmembrane glycoprotein that belongs to the Eph receptor family of tyrosine kinase receptors. EphB2 serves as a cell surface receptor tyrosine kinase for membrane-anchored ligands referred to as type B ephrins (ephrin-B). The EphB2 receptor can bind to ephrin-B1, ephrin-B2, and ephrin-B3. Transmembrane ephrin-B family members are key regulators of embryogenesis including development of the nervous and vascular systems. The EphB2 receptor functions as a chemodirectant in regulating cellular migration. EphB2/ephrin-B interactions orchestrate cell positioning by regulating cellular adhesion and repulsion during development, thereby influencing cell fate, morphogenesis and organogenesis. Signaling can occur in a forward pathway when the EphB2 receptor tyrosine kinase is activated by bound ligand and in a reverse pathway when transmembrane ephrin-B ligands are activated by EphB2 receptor-mediated crosslinking. In the adult body, Eph receptor signaling plays major roles in regulating the architecture and physiology of different tissues under normal as well as disease conditions such as cancer. Ephrin-B1 and ephrin-B2 levels are upregulated in the vasculature during inflammation. Ephrin-B2 Molecules that are localized to the luminal endothelial surface can signal through the EphB2 which is expressed by monocytes. This interaction promotes monocyte differentiation into proinflammatory macrophages. In the intestinal epithelium, EphB2/ephrin-B interactions regulate both cell positioning and tumor progression. The differential expression patterns of EphB2 allows for the detection and isolation of various intestinal epithelial cell types. These include intestinal stem cells (ISCs) which express high levels of EphB2. The 2H9 antibody reportedly blocks the interaction of EphB2 with ephrin ligands and crossreacts with mouse EphB2. Flow cytometric analysis of EphB2 expression on human Colo-205 cells. Cells from the human COLO 205 (Colorectal adenocarcinoma, ATCC CCL-222) cell line were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 550878, dashed line histogram) or Purified Mouse Anti-Human EphB2 antibody (Cat. No. 564698, solid line histogram). The cells were washed and counterstained with PE Goat Anti-Mouse Ig (Cat. No. 550589). The fluorescence histograms showing EphB2 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Immunohistochemical analysis of human EphB2 expression in human placenta. Acetone-fixed frozen human placenta sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat No.550878, Left Image) or Purified Mouse Anti-Human EphB2 antibody (Right Image). A three-step staining procedure that employed Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification:20x. 564698 Rev. 1 Page 1 of 2

BD Pharmingen™ Purified Mouse Anti-Human EphB2 - Purified - Clone 2H9 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human EphB2 Recombinant Protein

Clone: 2H9

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-EPH Receptor B2 antibody (ABIN2689499)

Jung, Sato, Merlos-Suárez, Barriga, Iglesias, Rossell, Auer, Gallardo, Blasco, Sancho, Clevers, Batlle: "Isolation and in vitro expansion of human colonic stem cells." in: Nature medicine, Vol. 17, Issue 10, pp. 1225-7, (2011) (PubMed).

Merlos-Suárez, Barriga, Jung, Iglesias, Céspedes, Rossell, Sevillano, Hernando-Momblona, da Silva-Diz, Muñoz, Clevers, Sancho, Mangues, Batlle: "The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse." in: Cell stem cell, Vol. 8, Issue 5, pp. 511-24, (2011) (PubMed).

Foster, Gordon, Cardenas, Veiga-Fernandes, Makinen, Grigorieva, Wilkinson, Blackburn, Richie, Manley, Adams, Kioussis, Coles: "EphB-ephrin-B2 interactions are required for thymus migration during organogenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 30, pp. 13414-9, (2010) (PubMed).

Mao, Luis, Ross, Silva, Tan, Crowley, Chui, Franz, Senter, Koeppen, Polakis: "EphB2 as a therapeutic antibody drug target for the treatment of colorectal cancer." in: Cancer research, Vol. 64, Issue 3, pp. 781-8, (2004) (PubMed).

Target Details for EPH Receptor B2

Target: EPH Receptor B2 (EPHB2)

Alternative Name: EphB2 (EPHB2 Products)

Synonyms: CAPB antibody, DRT antibody, EK5 antibody, EPHT3 antibody, ERK antibody, Hek5 antibody, PCBC antibody, Tyro5 antibody, ERK2 antibody, ERT1 antibody, MAPK2 antibody, P42MAPK antibody, PRKM1 antibody, PRKM2 antibody, p38 antibody, p40 antibody, p41 antibody, p41mapk antibody, RGD1564232 antibody, Cek5 antibody, Drt antibody, ETECK antibody, Erk antibody, Nuk antibody, Prkm5 antibody, Qek5 antibody, Sek3 antibody, cek5 antibody, ephb2 antibody, xEphB2 antibody, si:ch73-160e9.1 antibody, tpx2 antibody, wu:fb09e10 antibody, wu:fc64a03 antibody, wu:fi18g12 antibody, zgc:112296 antibody, EPH receptor B2 antibody, mitogen-activated protein kinase 1 antibody, Eph receptor B2 antibody, EPH receptor B2 L homeolog antibody, eph receptor B2a antibody, eph receptor B2b antibody, EPHB2 antibody, MAPK1 antibody, Ephb2 antibody, ephb2.L antibody, ephb2a antibody, ephb2b antibody

Background: Synonyms: EPHB2, EphB2R, TYRO5, CAPB, DRT, EK5, EPHT3, EPTH3, HEK5, PCBC

Pathways: RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins