IL12 antibody

Catalog No. ABIN2689730

This Rat Monoclonal antibody specifically detects IL12 in ELISA. It exhibits reactivity toward Mouse and has been mentioned in 3+ publications.

Quick Overview for IL12 antibody (ABIN2689730)

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL12 antibody is un-conjugated

Application: ELISA

Clone: C15-6

Product Details anti-IL12 Antibody

Brand: BD Pharmingen™

Characteristics: The C15.6 antibody reacts with both free and complexed (homodimer p80 and heterodimer p70) forms of the p40 subunit of mouse interleukin-12 (IL-12). The immunogen used to generate the C15.6 hybridoma was recombinant mouse IL-12 p70 protein. p40 has also been described as a subunit of IL-23 and thus it is possible that the C15.6 antibody will crossreact with IL-23. This antibody is routinely tested by ELISA capture. Other applications were tested during antibody development only or reported in the literature. Mouse IL-12p40 standard curve.

BD Pharmingen™ Purified Rat Anti-Mouse IL-12 p40/p70 - Purified - Clone C15.6 - Isotype Rat IgG1 - Reactivity Ms - 1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: CHO-expressed recombinant mouse IL-12 p70 protein

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL12 antibody (ABIN2689730)

Oppmann, Lesley, Blom, Timans, Xu, Hunte, Vega, Yu, Wang, Singh, Zonin, Vaisberg, Churakova, Liu, Gorman, Wagner, Zurawski, Liu, Abrams, Moore, Rennick, de Waal-Malefyt, Hannum, Bazan, Kastelein: "Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12." in: Immunity, Vol. 13, Issue 5, pp. 715-25, (2001) (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Wysocka, Kubin, Vieira, Ozmen, Garotta, Scott, Trinchieri: "Interleukin-12 is required for interferon-gamma production and lethality in lipopolysaccharide-induced shock in mice." in: European journal of immunology, Vol. 25, Issue 3, pp. 672-6, (1995) (PubMed).

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12 p40,p70

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome