ENPEP antibody (Biotin)

Catalog No. ABIN2689818

There are 16+ publications for this product available. The Rat Monoclonal anti-ENPEP antibody is suitable to detect ENPEP in samples from Mouse. It has been validated for FACS.

Quick Overview for ENPEP antibody (Biotin) (ABIN2689818)

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ENPEP antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 6C3

Product Details anti-ENPEP Antibody (Biotin)

Characteristics: The 6C3 antibody reacts with an epitope of the 6C3/BP-1 (Ly-51) glycoprotein cell-surface differentiation antigen, which was originally identified on pre-B cell lymphomas (spontaneous and chemical- or retrovirus- transformed, in vitro and in vivo). 6C3/BP-1 is a homodimer cell-surface glycoprotein with 140- kDa subunits which has been identified to possess aminopeptidase A (APA) activity. The same antigen is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoieses, on thymic dendritic cells and cortical epithelial cells, and on a wide variety of mouse and rat tissues known to possess APA activity. Subsets of normal bone marrow pre-B and B lymphocytes express low levels of Ly-51, which is rapidly up-regulated on the pre-B cells in the presence of IL-7. A role for the 6C3/BP-1 Molecule in the IL-7-driven proliferation of B cell precursors has been postulated. However, B-cell abnormalities were not detected in Ly-51-deficient mice. Mature B lymphocytes, thymocytes, peripheral T lymphocytes, erythroid cells, and myeloid cells (with the exception of thymic dendritic cells) do not express Ly-51. The 6C3 antibody can be used to identify cortical epithelium in frozen sections of thymuses from normal, SCID, and TCR-transgenic mice. It is possible that the low level of 6C3/BP-1 antigen detected, by flow cytometry, on some thymocytes may be passively adsorbed from adjacent epithelial cells during preparation of the cell suspensions.

BD Pharmingen™ Biotin Rat Anti-Mouse Ly-51 - Biotin - Clone 6C3 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: C57L mouse Pre-B lymphhoma cell line L1-2

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-ENPEP antibody (Biotin) (ABIN2689818)

Lin, Taniuchi, Kitamura, Wang, Kearney, Watanabe, Cooper: "T and B cell development in BP-1/6C3/aminopeptidase A-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 10, pp. 4681-7, (1998) (PubMed).

Goverman, Brabb, Huseby, Farr: "TCR signaling regulates thymic organization: lessons from TCR-transgenic mice." in: Immunology today, Vol. 18, Issue 5, pp. 204-8, (1997) (PubMed).

Welch: "Regulation of B cell precursor proliferation by aminopeptidase A." in: International immunology, Vol. 7, Issue 5, pp. 737-46, (1995) (PubMed).

Wu, Vremec, Ardavin, Winkel, Süss, Georgiou, Maraskovsky, Cook, Shortman: "Mouse thymus dendritic cells: kinetics of development and changes in surface markers during maturation." in: European journal of immunology, Vol. 25, Issue 2, pp. 418-25, (1995) (PubMed).

Wang, Cooper: "Histidine residue in the zinc-binding motif of aminopeptidase A is critical for enzymatic activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 4, pp. 1222-6, (1993) (PubMed).

Surh, Ernst, Sprent: "Growth of epithelial cells in the thymic medulla is under the control of mature T cells." in: The Journal of experimental medicine, Vol. 176, Issue 2, pp. 611-6, (1992) (PubMed).

Welch, Burrows, Namen, Gillis, Cooper: "Bone marrow stromal cells and interleukin-7 induce coordinate expression of the BP-1/6C3 antigen and pre-B cell growth." in: International immunology, Vol. 2, Issue 8, pp. 697-705, (1991) (PubMed).

Wu, Li, Cooper, Pierres, Gorvel: "Aminopeptidase A activity of the murine B-lymphocyte differentiation antigen BP-1/6C3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 2, pp. 676-80, (1991) (PubMed).

Hardy, Carmack, Shinton, Kemp, Hayakawa: "Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow." in: The Journal of experimental medicine, Vol. 173, Issue 5, pp. 1213-25, (1991) (PubMed).

Sherwood, Weissman: "The growth factor IL-7 induces expression of a transformation-associated antigen in normal pre-B cells." in: International immunology, Vol. 2, Issue 5, pp. 399-406, (1991) (PubMed).

Ramakrishnan, Wu, Yue, Cooper, Rosenberg: "BP-1/6C3 expression defines a differentiation stage of transformed pre-B cells and is not related to malignant potential." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 5, pp. 1603-8, (1990) (PubMed).

Wu, Lahti, Air, Burrows, Cooper: "Molecular cloning of the murine BP-1/6C3 antigen: a member of the zinc-dependent metallopeptidase family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 3, pp. 993-7, (1990) (PubMed).

Wu, Tidmarsh, Welch, Pierce, Weissman, Cooper: "The early B lineage antigen BP-1 and the transformation-associated antigen 6C3 are on the same molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 10, pp. 3303-8, (1989) (PubMed).

Adkins, Tidmarsh, Weissman: "Normal thymic cortical epithelial cells developmentally regulate the expression of a B-lineage transformation-associated antigen." in: Immunogenetics, Vol. 27, Issue 3, pp. 180-6, (1988) (PubMed).

Morse, Tidmarsh, Holmes, Frederickson, Hartley, Pierce, Langdon, Dailey, Weissman: "Expression of the 6C3 antigen on murine hematopoietic neoplasms. Association with expression of abl, ras, fes, src, erbB, and Cas NS-1 oncogenes but not with myc." in: The Journal of experimental medicine, Vol. 165, Issue 3, pp. 920-5, (1987) (PubMed).

Pillemer, Whitlock, Weissman: "Transformation-associated proteins in murine B-cell lymphomas that are distinct from Abelson virus gene products." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 14, pp. 4434-8, (1984) (PubMed).

Target Details for ENPEP

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Alternative Name: Ly-51

Target Type: Chemical

Background: Synonyms: 6C3/BP-1 Antigen

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones